B Lymphocyte Specification Is Preceded by Extensive Epigenetic Priming in Multipotent Progenitors

Abstract B lymphocyte development is dependent on the interplay between chromatin landscape and lineage-specific transcription factors. It has been suggested that lineage commitment associated with major changes in nuclear environment, proposing a critical role for factors formation of epigenetic landscape. In this report, we have used chromosome conformation capture combination assay transposase-accessible sequencing analysis to enable highly efficient annotation both proximal distal transcriptional control elements genes activated specification mice. A large majority these were annotated at least one regulatory element an accessible configuration multipotent progenitors. Furthermore, binding sites key regulators specification, EBF1 PAX5, occurred already regions. did, however, cause dynamic change accessibility was increase promoter–enhancer interactions. Our data unravel extensive priming lineage-restricted provide insight into cell specification.